The NASA ER-2 Navigation Data ALOFT dataset contains information recorded by the onboard navigation and data collection systems of the NASA ER-2 high-altitude research aircraft. In addition to typical navigation data (e.g., date, time, latitude/longitude, and altitude) it also contains outside meteorological parameters such as wind speed, wind direction, and temperature. These data were collected during the Airborne Lightning Observatory for FEGS and TGFs (ALOFT) field campaign. ALOFT aimed to observe TGFs in one of the most TGF-intense regions on the planet, to observe gamma-ray glows in thunderstorms and their relation to TGFs, to perform ISS LIS and GLM validation using improved suborbital instrumentation, to evaluate new design concepts for next-generation spaceborne lightning mappers, and to make combined microwave and lightning measurements of tropical convection from a suborbital platform. The ALOFT navigation dataset files are available from June 15, 2023, through July 31, 2023, in ASCII format.