This data set, LBA-ECO LC-22 Land Cover from MODIS Vegetation Indices, Mato Grosso, Brazil, provides land cover classifications for Mato Grosso, Brazil, for the years 2000-2001 and 2003-2004. The classifications were derived from annual vegetation phenology information from a time series of Collection 4, 16-day MODerate Resolution Imaging Spectroradiometer (MODIS) Normalized Difference Vegetation Index (NDVI), and the Enhanced Vegetation Index (EVI) vegetation data, at 250-m resolution. A decision tree classifier was trained using field observations and Landsat TM data of land cover from 2003-2004 to identify seven land-cover classes. The classifier was applied to the 2000-2001 and 2003-2004 MODIS ENVI and EVI data. There are two GeoTIFF (.tif) files with this data set.